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Antibodies

CTCF antibody (pAb)

Aliases: MRD21
Catalog No: 61311 Format: 100 µl $420 Buy Now
Catalog No: 61312 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:120kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Electrophoretic Mobility Shift Assay Validated Immunocytochemistry Validated

for CTCF antibody (pAb)Application Notes


Validated Applications:
ChIP: 2 - 8 µl per ChIP
ChIP-Seq: 5 µl each
ICC/IF: 1:2,000 dilution
WB: 1:500- 1:2,500 dilution

Published Applications:
ChIP
EMSA

for CTCF antibody (pAb)Immunogen

This CTCF antibody was raised against a peptide within the N-terminal region of human CTCF.

for CTCF antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CTCF antibody (pAb)References

 
CTCF antibody (pAb) tested by ChIP-chip.

CTCF antibody (pAb) tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human B cell lymphoma cell line (4.5 million cells) and 5 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows CTCF binding across a 1.2 million bp region on chromosome 7.

CTCF antibody (pAb) tested by ChIP.

CTCF antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 8 µl of CTCF antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the SCN4A gene (site A or site B). Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG (arbitrarily assigned a value of 1) using the ddCT method.

CTCF antibody (pAb) tested by immunofluorescence.

CTCF antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with CTCF antibody at a 1:2,000 dilution.

CTCF antibody (pAb) tested by Western blot.

CTCF pAb tested by Western blot.
CTCF detection by Western blot. The analysis was performed using 30 µg HeLa nuclear cell extract and CTCF at a 1:500 dilution.

for CTCF antibody (pAb)Background

CTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.

for CTCF antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CTCF antibody.

for CTCF antibody (pAb)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Negative Control Primer Set 1 (Cat. No. 71011) has been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with CTCF antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CTCF antibody (pAb).

for CTCF antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CTCF antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CTCF antibody (pAb)
 

Data Thumbnails

CTCF antibody (pAb) tested by ChIP-chip.

ChIP-chip of CTCF pAb.
(Click image to enlarge and see details.)

CTCF antibody (pAb) tested by ChIP.

ChIP of CTCF pAb.
(Click image to enlarge and see details.)

CTCF antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of CTCF pAb.
(Click image to enlarge and see details.)

CTCF antibody (pAb) tested by Western blot.

Western blot of CTCF pAb.
(Click image to enlarge and see details.)