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Antibodies

DNMT3A dimethyl Lys44 antibody (pAb)

Aliases: DNA Methyltransferase 3a, MmuIIIA
Catalog No: 61323 Format: 100 µl $410 Buy Now
Catalog No: 61324 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:120kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for DNMT3A dimethyl Lys44 antibody (pAb)Application Notes


Published Applications:
WB
See references for more information. Individual optimization may be required.

for DNMT3A dimethyl Lys44 antibody (pAb)Immunogen

This antibody was raised against a peptide including dimethyl Lys 44 of mouse DNMT3A (DNA methyltransferase 3 alpha)

for DNMT3A dimethyl Lys44 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for DNMT3A dimethyl Lys44 antibody (pAb)References

for DNMT3A dimethyl Lys44 antibody (pAb)Background

DNMT3ADNA methyltransferase proteins such as DNMT3A (or DNMT 3A) are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression.

Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells. Lys44 is dimethylated by G9a/GLP and is recognized by the chromo-domain containing protein MPP8.

for DNMT3A dimethyl Lys44 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DNMT3A dimethyl Lys44 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DNMT3A dimethyl Lys44 antibody (pAb)