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Antibodies

Tada2a antibody (mAb)

Clone: 2AD-2A1
Aliases: ADA2, ADA2A, KL04P, hADA2, TADA2L
Catalog No: 61333 Format: 100 µg $410 Buy Now
Catalog No: 61334 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:54 kDa
Reactivity:Human

Applications

Immunoprecipitation Validated

for Tada2a antibody (mAb) (Clone 2AD-2A1)Application Notes


Published Applications:
IP
See references for more information. Individual optimization may be required.

for Tada2a antibody (mAb) (Clone 2AD-2A1)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 1-17 of human Tada2a.

for Tada2a antibody (mAb) (Clone 2AD-2A1)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Tada2a antibody (mAb) (Clone 2AD-2A1)References

for Tada2a antibody (mAb) (Clone 2AD-2A1)Background

Tada2a (transcriptional adaptor 2A) is a component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a distant site (By similarity). Binds double-stranded DNA. Binds dinucleosomes, probably at the linker region between neighboring nucleosomes. Plays a role in chromatin remodeling.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Tada2a antibody (mAb).

for Tada2a antibody (mAb) (Clone 2AD-2A1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Tada2a antibody (mAb) (Clone 2AD-2A1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Tada2a antibody (mAb)