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Antibodies

Supt3h antibody (mAb)

Clone: 1SP-2C10
Aliases: Suppressor of Ty 3 homolog (S. cerevisiae), SPT3, SPT3L
Catalog No: 61343 Format: 100 µg $410 Buy Now
Catalog No: 61344 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:38 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated

for Supt3h antibody (mAb) (Clone 1SP-2C10)Application Notes


Published Applications:
ChIP
IP
WB
See references for more information. Individual optimization may be required.

for Supt3h antibody (mAb) (Clone 1SP-2C10)Immunogen

This antibody was raised against a peptide corresponding to amino acid residue(s) 10-27 of human Supt3h.

for Supt3h antibody (mAb) (Clone 1SP-2C10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Supt3h antibody (mAb) (Clone 1SP-2C10)References

for Supt3h antibody (mAb) (Clone 1SP-2C10)Background

Supt3h (suppressor of Ty 3 homolog (S. cerevisiae)) is a probable transcriptional activator and functions as a component in multiple histone acetyltransferase (HAT) complexes such as STAGA (SPT3-TAF-GCN5 acetylase), PCAF (p300/CBP associated factor), and TFTC (TBP-free TAF complex).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Supt3h antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Supt3h antibody (mAb).

for Supt3h antibody (mAb) (Clone 1SP-2C10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Supt3h antibody (mAb) (Clone 1SP-2C10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Supt3h antibody (mAb)