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Antibodies

OGT/O-GlcNAc transferase antibody (pAb)

Aliases: HRNT1, O-GLCNAC
Catalog No: 61355 Format: 100 µl $420 Buy Now
Catalog No: 61356 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:120 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for OGT/O-GlcNAc transferase antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 µl per ChIP
ChIP-Seq & ChIP-chip: 5 µl each
WB: 1:500- 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for OGT/O-GlcNAc transferase antibody (pAb)Immunogen

This antibody was raised against a recombinant protein corresponding to the N-terminal region of human OGT.

for OGT/O-GlcNAc transferase antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for OGT/O-GlcNAc transferase antibody (pAb)References

 
OGT/O-GlcNAc transferase antibody (pAb) tested by ChIP-Seq.

OGT/O-GlcNAc transferase (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million primary mouse T cells and 5 µl of OGT antibody. ChIP DNA was sequenced on the Illumina HiSeq and 14 million sequence tags were mapped to identify OGT binding sites. The image on the left shows many OGT binding sites within a 25 million bp region on mouse chromosome 8. The image on the right shows that binding sites are localized to gene promoters as expected based on the known interaction with the H3K4 methylating COMPASS complex.

OGT/O-GlcNAc transferase antibody (pAb) tested by Western blot.

OGT/O-GlcNAc transferase (pAb) tested by Western blot.
Detection of OGT/O-GlcNAc transferase by Western blot. The analysis was performed using 30 µg HeLa whole-cell extract and OGT/O-GlcNAc transferase (pAb) at a 1:500 dilution.

for OGT/O-GlcNAc transferase antibody (pAb)Background

OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression.

for OGT/O-GlcNAc transferase antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for OGT/O-GlcNAc transferase antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with OGT/O-GlcNAc transferase antibody (pAb).

for OGT/O-GlcNAc transferase antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for OGT/O-GlcNAc transferase antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

OGT/O-GlcNAc transferase antibody (pAb)
 

Data Thumbnails

OGT/O-GlcNAc transferase antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of OGT/O-GlcNAc transferase pAb.
(Click image to enlarge and see details.)

OGT/O-GlcNAc transferase antibody (pAb) tested by Western blot.

Western blot of OGT/O-GlcNAc transferase pAb.
(Click image to enlarge and see details.)