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Antibodies

Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)

Catalog No: 61393 Format: 100 µl $410 Buy Now
Catalog No: 61394 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:None
Host:Rabbit
Molecular Weight:8 kDa
Reactivity:Human

Applications

Dot Blot Validated

for Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)Application Notes


Validated Applications:
DB: 1:500 - 1:2,000 dilution

for Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)Immunogen

This antibody was raised against a synthetic peptide containing biotin-Lys 12 of human Histone H4.

for Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)References

 
Histone H4K8K12K16 (pan-biotinylated) antibody (pAb) tested by dot blot analysis.

Histone H4K8K12K16 (pan-biotinylated) pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4K8K12K16 (pan-biotinylated) antibody. Modified and unmodified peptide were spotted onto the membrane and probed with the antibody.
Lane 1: Peptide biotinylated at lysine 8 of Histone H4.
Lane 2: Peptide biotinylated at lysine 12 of Histone H4.
Lane 3: Peptide biotinylated at lysine 16 of Histone H4.
Lane 4: Unmodified peptide corresponding to amino acids 1-20 of Histone H4.

for Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation, biotinylation and SUMOylation; these modifications play a major role in regulating gene expression.

The biotinylation of Histone H4 on Lys8, Lys12 and Lys16 has been reported to be enriched in repeat regions and may participate in repression of transcriptionally competent genes.

for Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)
 

Data Thumbnails

Histone H4K8K12K16 (pan-biotinylated) antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4K8K12K16 (pan-biotinylated) pAb.
(Click image to enlarge and see details.)