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Antibodies

SUV420H1 antibody (pAb)

Aliases: CGI85, KMT5B, CGI-85
Catalog No: 61415 Format: 100 µl $410 Buy Now
Catalog No: 61416 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:100 kDa
Reactivity:Human

Applications

Western Blot Validated

for SUV420H1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Published Applications:
WB

for SUV420H1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human SUV420H1.

for SUV420H1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for SUV420H1 antibody (pAb)References

 
SUV420H1 antibody (pAb) tested by Western blot.

SUV420H1 antibody (pAb) tested by Western blot.
Nuclear extract of Hep G2 cells (30 µg) probed with SUV420H1 antibody (pAb) at a dilution of 1:500.

for SUV420H1 antibody (pAb)Background

SUV420H1 (suppressor of variegation 4-20 homolog 1) is a histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity).

for SUV420H1 antibody (pAb)Positive Control

Active Motif's Hep G2 nuclear extract (Catalog No. 36011) can be used as a positive control for SUV420H1 antibody.

for SUV420H1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SUV420H1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SUV420H1 antibody (pAb)
 

Data Thumbnails

SUV420H1 antibody (pAb) tested by Western blot.

Western blot of SUV420H1 pAb.
(Click image to enlarge and see details.)