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Antibodies

OGA/O-GlcNAcase antibody (pAb)

Aliases: MGEA5, OGA, MEA5, NCOAT
Catalog No: 61425 Format: 100 µl $410 Buy Now
Catalog No: 61426 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:115 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for OGA/O-GlcNAcase antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for OGA/O-GlcNAcase antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human OGA/ O-GlcNAcase.

for OGA/O-GlcNAcase antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for OGA/O-GlcNAcase antibody (pAb)References

 
OGA/O-GlcNAcase antibody (pAb) tested by immunoprecipitation.

OGA/O-GlcNAcase antibody (pAb) tested by Immunoprecipitation.
10 µl of OGA/O-GlcNAcase antibody was used to immunoprecipitate OGA/O-GlcNAcase from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the OGA/O-GlcNAcase antibody at a dilution of 1:1,000.

OGA/O-GlcNAcase antibody (pAb) tested by Western blot.

OGA/O-GlcNAcase pAb tested by Western blot.
OGA/O-GlcNAcase detection by Western blot. The analysis was performed using 20 µg HeLa whole cell extract and OGA/O-GlcNAcase at a 1:1,000 dilution.

for OGA/O-GlcNAcase antibody (pAb)Background

OGA / O GlcNAcase (O-linked N-acetylglucosamine (GlcNAc) -ase) is a glycosidase that removes O-linked N-acetylglucosamine (O-GlcNAc) modifications from serine and threonine residues on nuclear and cytoplasmic proteins. This dynamic modification is catalyzed by OGT and plays a role in homeostatic mechanisms and influences gene expression.

for OGA/O-GlcNAcase antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for OGA/O-GlcNAcase antibody.

for OGA/O-GlcNAcase antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for OGA/O-GlcNAcase antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

OGA/O-GlcNAcase antibody (pAb)
 

Data Thumbnails

OGA/O-GlcNAcase antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of OGA/O-GlcNAcase pAb.
(Click image to enlarge and see details.)

OGA/O-GlcNAcase antibody (pAb) tested by Western blot.

Western blot of OGA/O-GlcNAcase pAb.
(Click image to enlarge and see details.)