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Antibodies

Histone macroH2A1.2 antibody (mAb)

Clone: 14G7-H9
Catalog No: 61427 Format: 100 µl $410 Buy Now
Catalog No: 61428 Format: 10 µl $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Ascites
Host:Mouse
Molecular Weight:40 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for Histone macroH2A1.2 antibody (mAb)Application Notes


Validated Applications:
WB: 1:300 - 1:1,000 dilution
ICC/IF: 1:50 - 1:200 dilution

Published Applications:
ICC/IF
IHC
WB
See references for more information. Individual optimization may be required.

for Histone macroH2A1.2 antibody (mAb)Immunogen

Recombinant protein corresponding to amino acids 198-228 of human Histone macroH2A1.2.

for Histone macroH2A1.2 antibody (mAb)Buffer

Ascites containing 30% glycerol and 0.035% sodium azide

for Histone macroH2A1.2 antibody (mAb)References

 
Histone macroH2A1.2 antibody (mAb) tested by immunofluorescence.

Histone macroH2A1.2 antibody (mAb) tested by immunofluorescence.
Staining of macroH2A1.2 transfected HeLa cells with Histone macroH2A1.2 antibody (1:50 dilution, left panel) and DAPI (right panel).

Histone macroH2A1.2 antibody (mAb) tested by Western blot.

Histone macroH2A1.2 antibody (mAb) tested by Western blot.
U2OS cells mock transfected (lane 1) or transfected with tagged macroH2A1.1 (lane 2), macroH2A1.2 (lane 3) or macroH2A2 (lane 4) were probed with Histone macroH2A1.2 antibody at a dilution of 1:300. Band at 40kD is the endogenous macroH2A1.2.

for Histone macroH2A1.2 antibody (mAb)Background

Histone macroH2A1.2 is a histone H2A variant and one of the three members of the macroH2A family. The three macroH2A proteins contain the C-terminal 'macro' domain in addition to the H2A homologous region. This macro domain may bind poly ADP-ribose and other NAD+ metabolites. The macroH2A1.1 and macroH2A1.2 proteins are produced from alternatively spliced transcripts from the macroH2A1 gene while a second distinct macroH2A gene encodes macroH2A2. In mammals, macroH2A is preferentially localized to the inactive X chromosome and in general is proposed to play a role in repressing gene expression. In mice, macroH2A1 is required for the silencing of endogenous retroviruses.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone macroH2A1.2 antibody (mAb).

for Histone macroH2A1.2 antibody (mAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone macroH2A1.2 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone macroH2A1.2 antibody (mAb)
 

Data Thumbnails

Histone macroH2A1.2 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of Histone macroH2A1.2 mAb.
(Click image to enlarge and see details.)

Histone macroH2A1.2 antibody (mAb) tested by Western blot.

Western blot of Histone macroH2A1.2 mAb.
(Click image to enlarge and see details.)