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Antibodies

Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb)

Clone: 51Ta2H12
Catalog No: 61429 Format: 100 µg $410 Buy Now
Catalog No: 61430 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb) (Clone 51Ta2H12)Application Notes


Published Applications:
IP
ICC/IF
WB
DB
ELISA
See references for more information. Individual optimization may be required.

for Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb) (Clone 51Ta2H12)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residues 180-195 of human TAF10. The antibody recognizes H3 doubly modified at K9me2S10ph and/or K27me2S28ph.

for Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb) (Clone 51Ta2H12)Buffer

Purified IgG in 100mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.1 mM EDTA, 45 mM glycine, 0.05% sodium azide

for Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb) (Clone 51Ta2H12)References

for Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb) (Clone 51Ta2H12)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Histone H3 tails can be dimethylated on lysine (Kme2) and also phosphorylated on the adjacent serine (Sph) residue. H3K9me2S10ph and/or H3K28S29ph have been observed to occur during mitosis and may provide a specific chromatin conformation during chromatin condensation for cell division.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb).

for Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb) (Clone 51Ta2H12)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb) (Clone 51Ta2H12)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb)