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Antibodies

KDM1B / LSD2 antibody (pAb)

Catalog No: 61457 Format: 100 µl $410 Buy Now
Catalog No: 61458 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:93 kDa
Reactivity:Mouse

Applications

Western Blot Validated

for KDM1B / LSD2 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for KDM1B / LSD2 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of mouse KDM1B / LSD2.

for KDM1B / LSD2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for KDM1B / LSD2 antibody (pAb)References

 
KDM1B / LSD2 antibody (pAb) tested by Western blot.

KDM1B / LSD2 antibody (pAb) tested by Western blot.
Nuclear extract of NIH3T3 cells (40 µg) probed with KDM1B / LSD2 antibody (pAb) at a dilution of 1:500.

for KDM1B / LSD2 antibody (pAb)Background

KDM1B / LSD2 (Lysine (K)-Specific Demethylase 1B / Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity).

for KDM1B / LSD2 antibody (pAb)Positive Control

Active Motif's NIH/3T3 nuclear extract (Catalog No. 36020) can be used as a positive control for KDM1B / LSD2 antibody.

for KDM1B / LSD2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for KDM1B / LSD2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

KDM1B / LSD2 antibody (pAb)
 

Data Thumbnails

KDM1B / LSD2 antibody (pAb) tested by Western blot.

Western blot of KDM1B / LSD2 pAb.
(Click image to enlarge and see details.)