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Antibodies

DNMT1 antibody (mAb)

Clone: 2C1
Aliases: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN
Catalog No: 61467 Format: 100 µg $410 Buy Now
Catalog No: 61468 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:190 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated

for DNMT1 antibody (mAb) (Clone 2C1)Application Notes


Published Applications:
ChIP
IP
WB
See references for more information. Individual optimization may be required.

for DNMT1 antibody (mAb) (Clone 2C1)Immunogen

This antibody was raised against a full-length recombinant protein corresponding to human DNMT1.

for DNMT1 antibody (mAb) (Clone 2C1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for DNMT1 antibody (mAb) (Clone 2C1)References

for DNMT1 antibody (mAb) (Clone 2C1)Background

DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).

DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

for DNMT1 antibody (mAb) (Clone 2C1)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for DNMT1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with DNMT1 antibody (mAb).

for DNMT1 antibody (mAb) (Clone 2C1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DNMT1 antibody (mAb) (Clone 2C1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DNMT1 antibody (mAb)