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Antibodies

SMARCC2 / BAF170 antibody (pAb)

Aliases: SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily C, Member 2
Catalog No: 61471 Format: 100 µl $410 Buy Now
Catalog No: 61472 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:170 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for SMARCC2 / BAF170 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:2,000 dilution
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for SMARCC2 / BAF170 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of mouse SMARCC2 / BAF170.

for SMARCC2 / BAF170 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SMARCC2 / BAF170 antibody (pAb)References

 
SMARCC2 / BAF170 antibody (pAb) tested by immunofluorescence.

SMARCC2 / BAF170 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with SMARCC2 / BAF170 antibody at a 1:2,000 dilution.

SMARCC2 / BAF170 antibody (pAb) tested by Western blot.

SMARCC2 / BAF170 antibody (pAb) tested by Western blot.
Nuclear extract of K-562 cells (30 µg) probed with SMARCC2 / BAF170 antibody (1:500 dilution).

for SMARCC2 / BAF170 antibody (pAb)Background

SMARCC2 / BAF170 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily C, Member 2) is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

for SMARCC2 / BAF170 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for SMARCC2 / BAF170 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SMARCC2 / BAF170 antibody (pAb).

for SMARCC2 / BAF170 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SMARCC2 / BAF170 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SMARCC2 / BAF170 antibody (pAb)
 

Data Thumbnails

SMARCC2 / BAF170 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of SMARCC2 / BAF170 pAb.
(Click image to enlarge and see details.)

SMARCC2 / BAF170 antibody (pAb) tested by Western blot.

Western blot of SMARCC2 / BAF170 pAb.
(Click image to enlarge and see details.)