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Antibodies

CENP-A phospho Ser18 antibody (pAb)

Aliases: CenH3, CENPA
Catalog No: 61483 Format: 100 µl $410 Buy Now
Catalog No: 61484 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:16 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for CENP-A phospho Ser18 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:100 dilution
WB: 1:100 - 1:500 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Published Applications:
IF

for CENP-A phospho Ser18 antibody (pAb)Immunogen

This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A.

for CENP-A phospho Ser18 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CENP-A phospho Ser18 antibody (pAb)References

 
CENP-A phospho Ser18 antibody (pAb) tested by immunofluorescence.

CENP-A phospho Ser18 antibody (pAb) tested by immunofluorescence.
Detection of CENP-A phospho Ser18 by immunofluorescence. HeLa cells were stained with CENP-A phospho Ser18 antibody at a 1:100 dilution.
Green: CENP-A phospho Ser18 antibody (pAb)
Blue: DAPI

CENP-A phospho Ser18 antibody (pAb) tested by Western blot.

CENP-A phospho Ser18 antibody (pAb) tested by Western blot.
Top: Recombinant CENP-A (20 ng) was probed with CENP-A phospho Ser18 antibody (1:100 dilution) (A) or with anti-centromere antibody (ACA) (B). CENP-A phospho Ser18 antibody does not detect recombinant CENP-A.
Bottom: HeLa nuclear extract (20 µg) was probed with CENP-A phospho Ser18 antibody (1:250 dilution). Lane 1: CENP-A phospho-Ser18 antibody. Lane 2: anti-centromere antibody (ACA).

CENP-A phospho Ser18 antibody (pAb) tested by dot blot analysis.

CENP-A phospho Ser18 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of CENP-A phospho Ser18 antibody. Peptides corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:750. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: CENP-A phospho Ser 16 and phospho Ser18.
Lane 2: CENP-A unmodified.
Lane 3: CENP-A phospho Ser 16.
Lane 4: CENP-A phospho Ser 18.

for CENP-A phospho Ser18 antibody (pAb)Background

CENP-A (Centromere Protein A) is a Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. Recently, phosphorylation of CENP-A on Ser16 and Ser18 was reported and found to be important for chromosome segregation during mitosis.

for CENP-A phospho Ser18 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CENP-A antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CENP-A phospho Ser18 antibody (pAb).

for CENP-A phospho Ser18 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CENP-A phospho Ser18 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CENP-A phospho Ser18 antibody (pAb)
 

Data Thumbnails

CENP-A phospho Ser18 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of CENP-A phospho Ser18 pAb.
(Click image to enlarge and see details.)

CENP-A phospho Ser18 antibody (pAb) tested by Western blot.

Western blot of CENP-A phospho Ser18 pAb.
(Click image to enlarge and see details.)

CENP-A phospho Ser18 antibody (pAb) tested by dot blot analysis.

Dot blot of CENP-A phospho Ser18 pAb.
(Click image to enlarge and see details.)