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Antibodies

BRD3 antibody (pAb)

Aliases: ORFX, RING3L
Catalog No: 61489 Format: 100 µl $420 Buy Now
Catalog No: 61490 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:100 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for BRD3 antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq & ChIP-chip: 10 µl each
WB: 1:500- 1:2,000 dilution

Published Applications:
ChIP

for BRD3 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of mouse BRD3.

for BRD3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for BRD3 antibody (pAb)References

 
BRD3 antibody (pAb) tested by ChIP-Seq.

BRD3 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human H526 cells and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to show the expected promoter localization.

BRD3 antibody (pAb) tested by ChIP.

BRD3 antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human H526 cells and 10 µl BRD3 antibody. ChIP DNA was used in qPCR with the control primer or gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

BRD3 antibody (pAb) tested by Western blot.

BRD3 antibody (pAb) tested by Western blot.
Detection of BRD3 by Western blot analysis. Lane 1: Whole cell extract of HeLa cells (20 µg). Lane 2: Whole cell extract of NIH-3T3 cells. Both probed with BRD3 antibody (pAb) at a 1:500 dilution.

for BRD3 antibody (pAb)Background

BRD3 (Bromodomain Containing 3) is a member of the mammalian BET (Bromodomain Extra Terminal) family of proteins, binds hyperacetylated chromatin and has been reported to interact with acetylated Histone H4K5, Histone H4K12 and Histone H3K14. It plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with BRD3 antibody (pAb).

for BRD3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for BRD3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

BRD3 antibody (pAb)
 

Data Thumbnails

BRD3 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of BRD3 pAb.
(Click image to enlarge and see details.)

BRD3 antibody (pAb) tested by ChIP.

ChIP of BRD3 pAb.
(Click image to enlarge and see details.)

BRD3 antibody (pAb) tested by Western blot.

Western blot of BRD3 pAb.
(Click image to enlarge and see details.)