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Antibodies

STAT4 antibody (pAb)

Aliases: SLEB11
Catalog No: 61507 Format: 100 µl $410 Buy Now
Catalog No: 61508 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:86 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for STAT4 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for STAT4 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human STAT4.

for STAT4 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for STAT4 antibody (pAb)References

 
STAT4 antibody (pAb) tested by Western blot.

STAT4 antibody (pAb) tested by Western blot.
Nuclear extract of mouse testis (30 µg) probed with STAT4 antibody (pAb) at a dilution of 1:2,000.

for STAT4 antibody (pAb)Background

STAT4 (Signal Transducer And Activator Of Transcription 4) is involved in gene activation in response to IL-12, and is required for the development of the Th1 cell lineage. Both IL-12 and IFNα are capable of activating STAT4. Variants of the STAT4 gene are frequently associated with numerous auto-immune disorders.

for STAT4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for STAT4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

STAT4 antibody (pAb)
 

Data Thumbnails

STAT4 antibody (pAb) tested by Western blot.

Western blot of STAT4 pAb.
(Click image to enlarge and see details.)