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Antibodies

RING1 antibody (pAb)

Aliases: Ring Finger Protein 1, RNF1, RING1A
Catalog No: 61517 Format: 100 µl $410 Buy Now
Catalog No: 61518 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:50 kDa
Reactivity:Human

Applications

Western Blot Validated

for RING1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:250 - 1:500 dilution

for RING1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human RING1.

for RING1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RING1 antibody (pAb)References

 
RING1 antibody (pAb) tested by Western blot.

RING1 antibody (pAb) tested by Western blot.
Nuclear extract of Raji cells (25 µg) probed with RING1 antibody (pAb) at a dilution of 1:500.

for RING1 antibody (pAb)Background

RING1 (Ring Finger Protein 1) constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

for RING1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RING1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RING1 antibody (pAb)
 

Data Thumbnails

RING1 antibody (pAb) tested by Western blot.

Western blot of RING1 pAb.
(Click image to enlarge and see details.)