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Antibodies

NR3C1 antibody (pAb)

Aliases: GR, GCR, GRL, GCCR
Catalog No: 61519 Format: 100 µl $410 Buy Now
Catalog No: 61520 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:94 kDa
Reactivity:Human

Applications

Western Blot Validated

for NR3C1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for NR3C1 antibody (pAb)Immunogen

This antibody was raised against a peptide within human NR3C1.

for NR3C1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NR3C1 antibody (pAb)References

 
NR3C1 antibody (pAb) tested by Western blot.

NR3C1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (30 µg) probed with NR3C1 antibody (pAb) at a dilution of 1:500.

for NR3C1 antibody (pAb)Background

NR3C1 (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor)) is a receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation.

for NR3C1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NR3C1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NR3C1 antibody (pAb)
 

Data Thumbnails

NR3C1 antibody (pAb) tested by Western blot.

Western blot of NR3C1 pAb.
(Click image to enlarge and see details.)