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Antibodies

Histone H4K20ac antibody (mAb)

Clone: MABI 0420
Catalog No: 61531 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Dot Blot Validated

for Histone H4K20ac antibody (mAb) (Clone MABI 0420)Application Notes


Validated Applications:
ChIP: 2 - 5 µg per ChIP

for Histone H4K20ac antibody (mAb) (Clone MABI 0420)Immunogen

This antibody was raised against a synthetic peptide containing acetyl-lysine 20 of human Histone H4.

for Histone H4K20ac antibody (mAb) (Clone MABI 0420)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H4K20ac antibody (mAb) (Clone MABI 0420)References

 
Histone H4K20ac antibody (mAb) tested by ChIP.

Histone H4K20ac antibody (mAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 µg of Histone H4K20ac (mAb) or the equivalent amount of mouse IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H4K20ac (mAb) antibody specificity tested by peptide array analysis.

Histone H4K20ac antibody (mAb) specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20ac antibody (mAb) was applied at a dilution of 0.7 µg/ml to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

Histone H4K20ac antibody (mAb) tested by dot blot analysis.

Histone H4K20ac antibody (mAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4K20ac antibody (mAb) for acetyl-Lys20 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K20ac antibody (mAb) at a dilution of 2 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide. Lane 9: acetyl-Lys20 peptide. Lane 10: unmodified Lys20 peptide. Lane 11: acetyl-Lys31 peptide. Lane 12: unmodified Lys31 peptide.

for Histone H4K20ac antibody (mAb) (Clone MABI 0420)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4).

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4K20ac antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H4K20ac antibody (mAb).

for Histone H4K20ac antibody (mAb) (Clone MABI 0420)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4K20ac antibody (mAb) (Clone MABI 0420)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4K20ac antibody (mAb)
 

Data Thumbnails

Histone H4K20ac antibody (mAb) tested by ChIP.

ChIP of Histone H4K20ac mAb.
(Click image to enlarge and see details.)

Histone H4K20ac (mAb) antibody specificity tested by peptide array analysis.

Peptide Array analysis of Histone H4K20ac (mAb).
(Click image to enlarge and see details.)

Histone H4K20ac antibody (mAb) tested by dot blot analysis.

Dot blot of Histone H4K20ac mAb.
(Click image to enlarge and see details.)