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Antibodies

Histone H4K20me2 antibody (mAb)

Clone: MABI 0422
Catalog No: 61533 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Dot Blot Validated

for Histone H4K20me2 antibody (mAb) (Clone MABI 0422)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

for Histone H4K20me2 antibody (mAb) (Clone MABI 0422)Immunogen

This antibody was raised against a synthetic peptide containing dimethyl-lysine 20 of human Histone H4.

for Histone H4K20me2 antibody (mAb) (Clone MABI 0422)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H4K20me2 antibody (mAb) (Clone MABI 0422)References

 
Histone H4K20me2 antibody (mAb) tested by Western blot.

Histone H4K20me2 antibody (mAb) tested by Western blot.
Nuclear extract (20 µg) of HeLa cells probed with the Histone H4K20me2 antibody (mAb) at 2 µg/ml.

Histone H4K20me2 (mAb) antibody specificity tested by peptide array analysis.

Histone H4K20me2 antibody (mAb) specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me2 antibody (mAb) was applied at a dilution of 0.5 µg/ml to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

Histone H4K20me2 antibody (mAb) tested by dot blot analysis.

Histone H4K20me2 antibody (mAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4K20me2 antibody (mAb) for dimethyl-Lys20 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K20me2 antibody (mAb) at 2 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Top panel: Lane 1: unmodified Lys79 peptide Lane 2: monomethyl-Lys79 H4 peptide Lane 3: dimethyl-Lys79 H4 peptide Lane 4: trimethyl-Lys79 H4 peptide Lane 5: monomethyl-Lys20 H4 peptide Lane 6: dimethyl-Lys20 H4 peptide Lane 7: trimethyl-Lys20 H4 peptide Lane 8: acetyl-Lys20 peptide Lane 9: unmodified Lys20 peptide Bottom panel: Lane 1: monomethyl-Lys31 H4 peptide Lane 2: dimethyl-Lys31 H4 peptide Lane 3: trimethyl-Lys31 H4 peptide Lane 4: monomethyl-Lys44 H4 peptide Lane 5: dimethyl-Lys44 H4 peptide Lane 6: trimethyl-Lys44 H4 peptide.

for Histone H4K20me2 antibody (mAb) (Clone MABI 0422)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

for Histone H4K20me2 antibody (mAb) (Clone MABI 0422)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4K20me2 antibody (mAb) (Clone MABI 0422)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4K20me2 antibody (mAb)
 

Data Thumbnails

Histone H4K20me2 antibody (mAb) tested by Western blot.

Western blot of Histone H4K20me2 mAb.
(Click image to enlarge and see details.)

Histone H4K20me2 (mAb) antibody specificity tested by peptide array analysis.

Peptide Array analysis of Histone H4K20me2 (mAb).
(Click image to enlarge and see details.)

Histone H4K20me2 antibody (mAb) tested by dot blot analysis.

Dot blot of Histone H4K20me2 mAb.
(Click image to enlarge and see details.)