Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

FOXO4 antibody (pAb)

Aliases: AFX, AFX1, MLLT7
Catalog No: 61573 Format: 100 µl $410 Buy Now
Catalog No: 61574 Format: 10 µl $95 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:60 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for FOXO4 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for FOXO4 antibody (pAb)Immunogen

This antibody was raised against synthetic peptides containing human FOXO4.

for FOXO4 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for FOXO4 antibody (pAb)References

 
FOXO4 antibody (pAb) tested by Western blot.

FOXO4 antibody (pAb) tested by Western blot.
Detection of FOXO4 antibody by Western blot analysis. Cytoplasmic extract (20 µg) of mouse testis (lane 1), HeLa (lane 2), and Jurkat (lane 3). All probed with FOXO4 antibody at a dilution of 1:500.

for FOXO4 antibody (pAb)Background

FOXO4 (Forkhead Box O4) is a transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

for FOXO4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for FOXO4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

FOXO4 antibody (pAb)
 

Data Thumbnails

FOXO4 antibody (pAb) tested by Western blot.

Western blot of FOXO4 pAb.
(Click image to enlarge and see details.)