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Antibodies

FOXO3 antibody (pAb)

Aliases: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2
Catalog No: 61593 Format: 100 µl $410 Buy Now
Catalog No: 61594 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:85 kDa
Reactivity:Human

Applications

Western Blot Validated

for FOXO3 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for FOXO3 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human FOXO3.

for FOXO3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for FOXO3 antibody (pAb)References

 
FOXO3 antibody (pAb) tested by Western blot.

FOXO3 antibody (pAb) tested by Western blot.
Jurkat cytosolic cell extract (20 µg) probed with FOXO3 antibody at a dilution of 1:500.

for FOXO3 antibody (pAb)Background

FOXO3 (forkhead box protein O3) is a transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

for FOXO3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for FOXO3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

FOXO3 antibody (pAb)
 

Data Thumbnails

FOXO3 antibody (pAb) tested by Western blot.

Western blot of FOXO3 pAb.
(Click image to enlarge and see details.)