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Antibodies

TCF4 antibody (pAb)

Aliases: E2-2, ITF2, PTHS, SEF2, ITF-2, SEF-2, TCF-4, SEF2-1, SEF2-1A, SEF2-1B, SEF2-1D, bHLHb19
Catalog No: 61609 Format: 100 µl $410 Buy Now
Catalog No: 61610 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:85 kDa
Reactivity:Human

Applications

Western Blot Validated

for TCF4 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for TCF4 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human TCF4.

for TCF4 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for TCF4 antibody (pAb)References

 
TCF4 antibody (pAb) tested by Western blot.

TCF4 antibody (pAb) tested by Western blot.
Detection of TCF4 by Western blot. The analysis was performed using 25 µg SY-SY5Y whole-cell extract and TCF4 (pAb) at a 1:500 dilution.

for TCF4 antibody (pAb)Background

TCF4 (Transcription Factor 4) is a transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.

for TCF4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TCF4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TCF4 antibody (pAb)
 

Data Thumbnails

TCF4 antibody (pAb) tested by Western blot.

Western blot of TCF4 pAb.
(Click image to enlarge and see details.)