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Antibodies

PAX6 antibody (pAb)

Aliases: AN, AN2, FVH1, MGDA, WAGR, D11S812E
Catalog No: 61611 Format: 100 µl $410 Buy Now
Catalog No: 61612 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:52 kDa
Reactivity:Human

Applications

Western Blot Validated

for PAX6 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for PAX6 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human PAX6.

for PAX6 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PAX6 antibody (pAb)References

 
PAX6 antibody (pAb) tested by Western blot.

PAX6 antibody (pAb) tested by Western blot.
Detection of PAX6 by Western blot. The analysis was performed using 30 µg HEK293 nuclear extract and PAX6 (pAb) at a 1:500 dilution.

for PAX6 antibody (pAb)Background

PAX6 (Paired Box 6) is a transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.

for PAX6 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PAX6 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PAX6 antibody (pAb)
 

Data Thumbnails

PAX6 antibody (pAb) tested by Western blot.

Western blot of PAX6 pAb.
(Click image to enlarge and see details.)