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Antibodies

Histone H3R17me2aK18ac antibody (pAb)

Catalog No: 61613 Format: 100 µl $410 Buy Now
Catalog No: 61614 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Mouse, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Dot Blot Validated

for Histone H3R17me2aK18ac antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
WB: 1:500 - 1:1,000 dilution

for Histone H3R17me2aK18ac antibody (pAb)Immunogen

This antibody was raised against a synthetic peptide containing asymmetric dimethyl arginine 17 and acetyl lysine 18 of human Histone H3.

for Histone H3R17me2aK18ac antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3R17me2aK18ac antibody (pAb)References

 
Histone H3R17me2aK18ac antibody (pAb) tested by ChIP.

Histone H3R17me2aK18ac antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed with 5 x 106 cell equivalents of chromatin from a murine HAFTL pro-B cell line and 10 µl of Histone H3R17me2aK18ac antibody. ChIP DNA was used in qPCR with a negative control primer pair (Neg) or enhancer-specific primer pairs that are “off” in B cells (Cd14) or “on” in B cells (IL7r), as indicated. This data shows that the dual modification R17me2aK18ac on Histone H3 is enriched in active enhancers. The data are presented as Fold over Input.

Histone H3R17me2aK18ac antibody (pAb) tested by Western blot.

Histone H3R17me2aK18ac antibody (pAb) tested by Western blot.
Nuclear extract of Raji cells (20 µg per lane) was probed with H3R17me2aK18ac (pAb) at a 1:500 dilution.
    Lane 1: Untreated cells.
    Lane 2: Cells treated with sodium butyrate.

Histone H3R17me2aK18ac antibody (pAb) tested by dot blot analysis.

Histone H3R17me2aK18ac antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3R17me2aK18ac antibody for asymmetric dimethyl-arginine 17 and acetyl-lysine 18 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with H3R17me2aK18ac antibody at a 1:500 dilution. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: Peptide containing asymmetric dimethyl-Arg17 and acetyl-Lys18 of Histone H3 .
Lane 2: unmodified Histone H3 peptide.
Lane 3: Asymmetric dimethyl-Arg17 of Histone H3 peptide.
Lane 4: Acetyl-Lys18 of Histone H3 peptide.

for Histone H3R17me2aK18ac antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residues. Acetylation of histones occurs on lysine and is typically associated with activation of gene expression. Co-occurrence of two modifications adds an additional layer of complexity and regulation to the function of these modifications. Dual modification of H3R17me2aK18ac may play a role in enhancer function.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3R17me2aK18ac antibody (pAb).

for Histone H3R17me2aK18ac antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3R17me2aK18ac antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3R17me2aK18ac antibody (pAb)
 

Data Thumbnails

Histone H3R17me2aK18ac antibody (pAb) tested by ChIP.

ChIP of Histone H3R17me2aK18ac pAb.
(Click image to enlarge and see details.)

Histone H3R17me2aK18ac antibody (pAb) tested by Western blot.

Western blot of Histone H3R17me2aK18ac pAb.
(Click image to enlarge and see details.)

Histone H3R17me2aK18ac antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3R17me2aK18ac pAb.
(Click image to enlarge and see details.)