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Antibodies

Histone H3ac (pan-acetyl) antibody (pAb)

Catalog No: 61637 Format: 100 µg $410 Buy Now
Catalog No: 61638 Format: 10 µg $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Dot Blot Validated

for Histone H3ac (pan-acetyl) antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µg per ChIP
WB: 0.2 - 1 µg/ml dilution

for Histone H3ac (pan-acetyl) antibody (pAb)Immunogen

This antibody was raised against a peptide including acetyl-lysines contained in the N-terminal tail of human Histone H3.

for Histone H3ac (pan-acetyl) antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a sera version (Catalog No. 39139) of this antibody is also available.

for Histone H3ac (pan-acetyl) antibody (pAb)References

 
Histone H3ac (pan-acetyl) antibody (pAb) tested by ChIP.

H3ac (pan-acetyl) antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 13 µg of chromatin from both HeLa cells and 10 µg H3ac (pan-acetyl) antibody. ChIP DNA was used in qPCR with the gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Histone H3ac (pan-acetyl) antibody (pAb) tested by Western blot.

Histone H3ac (pan-acetyl) antibody (pAb) tested by Western blot.
Detection of acetylated Histone H3 by Western blot analysis using 20 µg HeLa nuclear extract and Histone H3ac (pan-acetyl) antibody at a 1 µg/ml dilution.
Lane 1: Nuclear extract of untreated HeLa cells.
Lane 2: Nuclear extract of HeLa cells treated with sodium butyrate.

Histone H3ac (pan-acetyl) antibody (pAb) tested by dot blot analysis.

Histone H3ac (pan-acetyl)antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3ac antibody. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 2 µg/ml. The amount of peptide (picomoles) spotted is indicated next to the row.
Lane 1: acetyl-Lys9 peptide. Lane 2: unmodified Lys9 peptide. Lane 3: acetyl-Lys14 peptide. Lane 4: unmodified Lys14 peptide. Lane 5: acetyl-Lys18 peptide. Lane 6: unmodified Lys18 peptide. Lane 7: acetyl-Lys23 peptide. Lane 8: unmodified Lys23 peptide. Lane 9: acetyl-Lys27 peptide. Lane 10: unmodified Lys27 peptide.

for Histone H3ac (pan-acetyl) antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3ac (pan-acetyl) antibody (pAb).

for Histone H3ac (pan-acetyl) antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3ac (pan-acetyl) antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3ac (pan-acetyl) antibody (pAb)
 

Data Thumbnails

Histone H3ac (pan-acetyl) antibody (pAb) tested by ChIP.

ChIP of Histone H3ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H3ac (pan-acetyl) antibody (pAb) tested by Western blot.

Western blot of Histone H3ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H3ac (pan-acetyl) antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)