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Antibodies

p53 antibody (pAb)

Aliases: TP53, BCC7, LFS1, TRP53
Catalog No: 61657 Format: 100 µl $410 Buy Now
Catalog No: 61658 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:53 kDa
Reactivity:Human

Applications

Western Blot Validated

for p53 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for p53 antibody (pAb)Immunogen

This antibody was raised against recombinant human p53 protein.

for p53 antibody (pAb)Buffer

Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.

for p53 antibody (pAb)References

 
p53 antibody (pAb) tested by Western blot.

p53 antibody (pAb) tested by Western blot.
Detection of p53 by Western blot. HEK293 nuclear extract (30 µg) probed with p53 antibody at a 1:500 dilution.

for p53 antibody (pAb)Background

p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, a ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.

for p53 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for p53 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

p53 antibody (pAb)
 

Data Thumbnails

p53 antibody (pAb) tested by Western blot.

Western blot of p53 pAb.
(Click image to enlarge and see details.)