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Antibodies

Aurora A antibody (mAb)

Clone: 35C1
Aliases: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, PPP1R47
Catalog No: 61709 Format: 100 µg $410 Buy Now
Catalog No: 61710 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:50 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Flow Cytometry Validated Immunocytochemistry Validated

for Aurora A antibody (mAb) (Clone 35C1)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Published Applications:
WB
IP
ICC/IF
FC

for Aurora A antibody (mAb) (Clone 35C1)Immunogen

This antibody was raised against full-length recombinant human Aurora A protein.

for Aurora A antibody (mAb) (Clone 35C1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Aurora A antibody (mAb) (Clone 35C1)References

 
Aurora A antibody (mAb) tested by Western blot.

Aurora A antibody (mAb) (Clone 35C1) tested by Western blot.
Aurora A antibody detection by Western blot. The analysis of Aurora A was performed using HeLa cytoplasmic cell extract (20µg) and Aurora A antibody at a 1 µg/ml dilution.

for Aurora A antibody (mAb) (Clone 35C1)Background

Aurora A (Aurora Kinase A) is a mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Aurora A antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Aurora A antibody (mAb).

for Aurora A antibody (mAb) (Clone 35C1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Aurora A antibody (mAb) (Clone 35C1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Aurora A antibody (mAb)
 

Data Thumbnails

Aurora A antibody (mAb) tested by Western blot.

Western blot of Aurora A mAb.
(Click image to enlarge and see details.)