Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

MINA antibody (pAb)

Aliases: ROX, MDIG, NO52, MINA53
Catalog No: 61713 Format: 100 µl $410 Buy Now
Catalog No: 61714 Format: 10 µl $95 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:55 kDa
Reactivity:Human

Applications

Western Blot Validated

for MINA antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for MINA antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human MINA.

for MINA antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
MINA antibody (pAb) tested by Western blot.

MINA antibody (pAb) tested by Western blot.
Detection of MINA by Western blot analysis. Positive detection of MINA in nuclear extracts (20 μg per lane) derived from Jurkat cells (lane 1) and Raji cells (lane 2) at a 1:500 dilution.

for MINA antibody (pAb)Background

MINA (MYC Induced Nuclear Antigen) is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated Lys-9 on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on His-39. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.

for MINA antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MINA antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MINA antibody (pAb)
 

Data Thumbnails

MINA antibody (pAb) tested by Western blot.

Western blot of MINA pAb.
(Click image to enlarge and see details.)