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Antibodies

MXD1 antibody (mAb)

Clone: 5F4
Aliases: MAD, MAD1, BHLHC58
Catalog No: 61739 Format: 100 µg $410 Buy Now
Catalog No: 61740 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:30 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated

for MXD1 antibody (mAb) (Clone 5F4)Application Notes


Published Applications:
ChIP
IP
WB
See reference for more information. Individual optimization may be required.

for MXD1 antibody (mAb) (Clone 5F4)Immunogen

This antibody was raised against a recombinant protein corresponding amino acids 6-221 of human MXD1.

for MXD1 antibody (mAb) (Clone 5F4)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MXD1 antibody (mAb) (Clone 5F4)References

for MXD1 antibody (mAb) (Clone 5F4)Background

MXD1 (MAX Dimerization Protein 1) is a transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5-CAC[GA]TG-3. MAD thus antagonizes MYC transcriptional activity by competing for MAX.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MXD1 antibody (mAb).

for MXD1 antibody (mAb) (Clone 5F4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MXD1 antibody (mAb) (Clone 5F4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MXD1 antibody (mAb)