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Antibodies

Histone H2Av antibody (mAb)

Clone: 10E9.D1
Catalog No: 61751 Format: 100 µg $410 Buy Now
Catalog No: 61752 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:15 kDa
Reactivity:Drosophila

Applications

ChIP Validated Western Blot Validated

for Histone H2Av antibody (mAb) (Clone 10E9.D1)Application Notes


Validated Applications:
ChIP: 5 µg per ChIP
WB: 0.5 - 2 µg/ml dilution

for Histone H2Av antibody (mAb) (Clone 10E9.D1)Immunogen

This antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster Histone variant H2Av.

for Histone H2Av antibody (mAb) (Clone 10E9.D1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
Histone H2Av antibody (mAb) tested by ChIP.

Histone H2Av antibody (mAb) tested by ChIP.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from Drosophila cells and 5 µg of H2Av antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Histone H2Av antibody (mAb) tested by Western blot.

Histone H2Av antibody (mAb) tested by Western blot.
Detection of Histone H2Av by Western blot. The analysis was performed using Schneider’s Drosophila L2 nuclear extract (20µg)and the Histone H2Av antibody at a dilution of 1µg/ml.

for Histone H2Av antibody (mAb) (Clone 10E9.D1)Background

The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2Av antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H2Av antibody (mAb).

for Histone H2Av antibody (mAb) (Clone 10E9.D1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2Av antibody (mAb) (Clone 10E9.D1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2Av antibody (mAb)
 

Data Thumbnails

Histone H2Av antibody (mAb) tested by ChIP.

ChIP of Histone H2Av mAb.
(Click image to enlarge and see details.)

Histone H2Av antibody (mAb) tested by Western blot.

Western blot of Histone H2Av mAb.
(Click image to enlarge and see details.)