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Antibodies

N6-Methyladenosine (m6A) antibody (mAb)

Clone: 17-3-4-1
Catalog No: 61755 Format: 100 µg $410 Buy Now
Catalog No: 61756 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:Not Applicable
Reactivity:Budding Yeast, Human, Other (Wide Range)

Applications

Immunoprecipitation Validated Dot Blot Validated

for N6-Methyladenosine (m6A) antibody (mAb) (Clone 17-3-4-1)Application Notes


Published Applications:
IP
DB
See references for more information. Individual optimization may be required.

for N6-Methyladenosine (m6A) antibody (mAb) (Clone 17-3-4-1)Immunogen

This antibody was raised against 6-Methyladenosine conjugated to BSA.

for N6-Methyladenosine (m6A) antibody (mAb) (Clone 17-3-4-1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for N6-Methyladenosine (m6A) antibody (mAb) (Clone 17-3-4-1)References

for N6-Methyladenosine (m6A) antibody (mAb) (Clone 17-3-4-1)Background

N6-Methyladenosine (m6A) ) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3’ UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use N6-Methyladenosine (m6A) antibody (mAb).

for N6-Methyladenosine (m6A) antibody (mAb) (Clone 17-3-4-1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for N6-Methyladenosine (m6A) antibody (mAb) (Clone 17-3-4-1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

N6-Methyladenosine (m6A) antibody (mAb)