Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

YY1 antibody (pAb)

Aliases: DELTA, NF-E1, UCRBP, INO80S, YIN-YANG-1
Catalog No: 61779 Format: 100 µl $410 Buy Now
Catalog No: 61780 Format: 10 µl $95 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:63 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated

for YY1 antibody (pAb)Application Notes


Validated Applications:
ChIP-Seq: 7 µl per ChIP
IP: 0.5 - 4 µl per IP
WB: 1:2,000 - 1:5,000 dilution

for YY1 antibody (pAb)Immunogen

This antibody was raised against a recombinant protein corresponding to amino acids 249-414 of human YY1.

for YY1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for YY1 antibody (pAb)References

 
YY1 antibody (pAb) tested by ChIP-Seq.

YY1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from HeLa cells and 7 µl (4 µg) YY1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 11.6 million sequence tags were mapped to identify YY1 binding sites.

YY1 antibody (pAb) tested by immunoprecipitation.

YY1 antibody (pAb) tested by Immunoprecipitation.
2 µl of YY1 antibody was used to immunoprecipitate YY1 from 250 µg of Raji nuclear cell extract (lane 2). 2 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the YY1 antibody at a dilution of 1:1,000.

YY1 antibody (pAb) tested by Western blot.

YY1 antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of Raji (Lane 1) and HeLa cells (Lane 2) probed with YY1 antibody at a 1:5,000 dilution.

for YY1 antibody (pAb)Background

YY1 (Yin Yang 1) is a transcription factor that belongs to the GLI-Kruppel class of zinc finger transcription factors and is ubiquitously expressed. YY1 is known to have a fundamental role in normal biologic processes such as embryogenesis, differentiation, replication and cellular proliferation. YY1 exerts its effects on genes involved in these processes via its ability to initiate, activate or repress transcription depending upon the context in which it binds. Since expression and function of YY1 are known to be intimately associated with progression through phases of the cell cycle, the physiologic significance of YY1 activity has been applied to models of tumor biology. It can repress and activate a number of gene promoters and direct histone deacetylases (HDACs) and histone acetyltransferases (HATs).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with YY1 antibody (pAb).

for YY1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for YY1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

YY1 antibody (pAb)
 

Data Thumbnails

YY1 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of YY1 pAb.
(Click image to enlarge and see details.)

YY1 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of YY1 pAb.
(Click image to enlarge and see details.)

YY1 antibody (pAb) tested by Western blot.

Western blot of YY1 pAb.
(Click image to enlarge and see details.)