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Antibodies

Histone H2A.J antibody (pAb)

Catalog No: 61793 Format: 100 µl $410 Buy Now
Catalog No: 61794 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Reactivity:Human, Mouse

Applications

Western Blot Validated Dot Blot Validated

for Histone H2A.J antibody (pAb)Application Notes


Validated Applications:
Western Blot: 1:500 - 1:2500
Dot Blot: 1:1000

for Histone H2A.J antibody (pAb)Immunogen

This antibody was raised against a peptide comprising amino acids 122-129 of human Histone H2A.J.

for Histone H2A.J antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
Histone H2A.J antibody (pAb) tested by Western blot.

Western blot of Histone H2A.J pAb.
20 µg Nuclear Extract of HeLa cells (Lane 1) or 40 µg mouse 3T3-L1 cells before (Lane 2) or following stimulation for 12 days with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) resulting in differentiation into adipocytes (Lane 3).
MW:14kD

Histone H2A.J antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H2A.J pAb.
Dot blot analysis was used to confirm the specificity of Histone H2A.J antibody. Lane 1: H2A.J peptide. Lane 2: H2A peptide comprised of a.a. 114-126 of human Histone H2A.

for Histone H2A.J antibody (pAb)Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, and is responsible for establishing higher-order chromatin structure. Histone H2A.J is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. Histone H2A.J, which is found only in mammals, is shown to be associated with senescence, accumulating in senescent fibroblasts and promoting expression of inflammatory genes. Therefore, H2A.J may contribute to chronic inflammation and age-related diseases.

for Histone H2A.J antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2A.J antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2A.J antibody (pAb)
 

Data Thumbnails

Histone H2A.J antibody (pAb) tested by Western blot.

Western blot of Histone H2A.J pAb.
(Click image to enlarge and see details.)

Histone H2A.J antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H2A.J pAb.
(Click image to enlarge and see details.)