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Recombinant Proteins & Enzymes

Recombinant SUV420H2 protein

Catalog No: 81013 Format: 20 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SUV420H2 proteinContents

Recombinant SUV420H2 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SUV420H2 proteinBackground

SUV420H2 (Suppressor Of Variegation 4-20 Homolog 2, also known as KMT5C) is a histone methyltransferase that specifically trimethylates nucleosomal histone H4 on lysine 20 (H4K20). H4K20me3 is a histone marker that represents epigenetic transcriptional repression. SUV420H2 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H2 is targeted to histone H3 via its interaction with RB1 family proteins.

for Recombinant SUV420H2 proteinApplication Notes

Recombinant SUV420H2 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 2 µg Recombinant Nucleosomes (H3.1) (Cat. 31466) were incubated with 0 µg (-), 0.1 µg (+), 0.2 µg (++), 0.4 µg (+++) SUV420H2 protein, in 30 µl reaction containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 3 hr at RT. Products run on a 12.5% SDS-PAGE gel were detected with anti-H4K20me2 (Cat. 39540) or anti-H4K20me3 (Cat. 39672). SUV420H2 only was used as negative control.

for Recombinant SUV420H2 proteinProtein Details

Recombinant SUV420H2 protein was expressed in E. coli cells as full length protein (accession number NP_116090.2) and contains an N-terminal GST tag with an observed molecular weight of 78.4 kDa.

 
Recombinant SUV420H2 protein.

Recombinant SUV420H2 protein

10% SDS-PAGE Coomassie staining

MW: 78.4 kDa

Purity: > 60% 

Recombinant SUV420H2 activity assay.

Recombinant SUV420H2 activity assay
Recombinant Nucleosomes (H3.1) in various amounts were incubated with SUV420H2 protein. Reaction products were run on SDS-PAGE gel and detected with anti-H4K20me2 or anti-H4K20me3. The activity of SUV420H2 with nucleosomes as substrate is better than with histone octamers as substrate. 

for Recombinant SUV420H2 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SUV420H2 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant SUV420H2 protein
 

Data Thumbnails

Recombinant SUV420H2 protein.

Recombinant SUV420H2 protein
(Click to enlarge and view details).

Recombinant SUV420H2 activity assay.

Recombinant SUV420H2 activity assay
(Click to enlarge and view details).