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Recombinant Proteins & Enzymes

Recombinant METTL11A (NTMT1) protein

Catalog No: 81029 Format: 20 µg $395 Buy Now
Catalog No: 81729 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant METTL11A (NTMT1) proteinContents

Recombinant METTL11A / NTMT1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant METTL11A (NTMT1) proteinBackground

METTL11A (Methyltransferase-Like Protein 11A) / NTMT1 (N-Terminal Xaa-Pro-Lys N-Methyltransferase 1), also known as NRMT1 or NTM1A, is a distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. It specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. METTL11A catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met- 1). It is also responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. And it is required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.

for Recombinant METTL11A (NTMT1) proteinApplication Notes

This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

N-terminal Methylation Assay Conditions: 3 µM CENP-A peptide (GPRRRSRKPEAPRRR) was incubated with 50 nM METTL11A protein in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 2 hours at room temperature. Activity was detected by MALDI-TOF.
* represents an uncertainty peak.

Capacity ability: 60 turnovers / enzyme molecule

for Recombinant METTL11A (NTMT1) proteinProtein Details

Recombinant METTL11A / NTMT1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_054783.2) with an N-terminal FLAG tag. The molecular weight of the protein is 27 kDa.

 
Recombinant METTL11A / NTMT1 protein gel

Recombinant METTL11A / NTMT1 protein gel
10% SDS-PAGE Coomassie staining
MW: 27 kDa
Purity: >80%

MALDI-TOF assay for METTL11A protein activity

MALDI-TOF assay for METTL11A protein activity
CENP-A peptide was incubated with METTL11A protein in reaction buffer for 2 hours at room temperature. Activity was detected by MALDI-TOF.
* represents an uncertainty peak. Capacity ability: 60 turnovers / enzyme molecule

for Recombinant METTL11A (NTMT1) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant METTL11A (NTMT1) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant METTL11A (NTMT1) protein
 

Data Thumbnails

Recombinant METTL11A / NTMT1 protein gel

Recombinant METTL11A / NTMT1 protein gel
(Click to enlarge and view details)

MALDI-TOF assay for METTL11A protein activity

MALDI-TOF assay for METTL11A protein activity
(Click to enlarge and view details)