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Recombinant Proteins & Enzymes

Histone H3K9me1 Peptide - biotinylated

Catalog No: 81045 Format: 100 µg $150 Buy Now
Expressed In: Synthetic Protein Species: N/A

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for Histone H3K9me1 Peptide - biotinylatedContents

100 µg of lyophilized powder.

for Histone H3K9me1 Peptide - biotinylatedBackground

Active Motif biotinylated histone peptides are suitable for use in a variety of binding, pull-down and enzymatic assays for the study of proteins that interact with histones and that read, write or erase histone modifications. 

for Histone H3K9me1 Peptide - biotinylatedApplication Notes

Spin vial briefly in microfuge. Reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot reconstituted peptide to avoid repeated freeze-thawing.

Reference: Fuchs, S.M. et. al. Curr Biol. 2011 Jan 11; 21(1): 53–58.

for Histone H3K9me1 Peptide - biotinylatedProtein Details

A synthetic peptide derived from Histone H3.1, amino acids 1-21, modified by methylation on lysine 9, with an added lysine residue containing a biotin moiety and a C-terminal amide group (CONH2). C-term biotinylated Lys residue is separated with two Gly (provide short spacer) and one Tyr (provides accurate UV quantification) from the rest of the peptide sequence. The presence of the lysine residue is for quantitation and does not affect peptide functionality.

Peptide Sequence: ARTKQTAR-Kme-STGGKAPRKQLA - GGYK(Biotin) - NH2

Calculated MW Mavg 2989.41
Observed Mavg 2901.17
Purity: 95% by HPLC and Mass Spectrometry.

 
Deconvoluted MALDI-TOF mass spectrum of biotinylated peptide (1-21 H3 histone amino acids).

Deconvoluted MALDI-TOF mass spectrum of biotinylated peptide (1-21 H3 histone amino acids).

for Histone H3K9me1 Peptide - biotinylatedStorage

Store lyophilized peptide at -20°C. Peptides in solution are stable for 6 months at -80°C.

 

Technical Data Sheet

Histone H3K9me1 Peptide - biotinylated
 

Data Thumbnails

Deconvoluted MALDI-TOF mass spectrum of biotinylated peptide (1-21 H3 histone amino acids).

Deconvoluted MALDI-TOF mass spectrum of biotinylated peptide (1-21 H3 histone amino acids).
(Click to enlarge and view details).