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Recombinant Proteins & Enzymes

Recombinant METTL11A (NTMT1) protein, His-Tag

Catalog No: 81062 Format: 100 µg $395 Buy Now
Catalog No: 81762 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant METTL11A (NTMT1) protein, His-TagContents

Recombinant METTL11A / NTMT1, His-tag protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol and 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant METTL11A (NTMT1) protein, His-TagBackground

METTL11A (Methyltransferase-Like Protein 11A) / NTMT1 (N-Terminal Xaa-Pro-Lys N-Methyltransferase 1), also known as NRMT1 or NTM1A, is a distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. It specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. METTL11A catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met- 1). It is also responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. And it is required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.

for Recombinant METTL11A (NTMT1) protein, His-TagApplication Notes

This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

N-terminal Methylation Assay: 3 µM CENP-A peptide (GPRRRSRKPEAPRRR) was incubated with 100 nM METTL11A, His tag protein in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 2 hours at room temperature. Activity was detected by MALDI-TOF. 

for Recombinant METTL11A (NTMT1) protein, His-TagProtein Details

Recombinant METTL11A / NTMT1, His-tag protein was expressed in E.coli cells as the full length protein (accession number NP_054783.2) with an N-terminal 6×His tag. The molecular weight of the protein is 28.9 kDa.

 
Recombinant METTL11A / NTMT1, His-tag , protein gel

Recombinant METTL11A / NTMT1, His-tag , protein gel
12.5% SDS-PAGE Coomassie staining
MW: 28.9 kDa
Purity: >95%

MALDI-TOF Assay for METTL11A activity

MALDI-TOF Assay for METTL11A activity
CENP-A peptide was incubated with METTL11A, His tag protein in reaction for 2 hours at room temperature. Activity was detected by MALDI-TOF. 

for Recombinant METTL11A (NTMT1) protein, His-TagStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant METTL11A (NTMT1) protein, His-TagGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant METTL11A (NTMT1) protein, His-Tag
 

Data Thumbnails

Recombinant METTL11A / NTMT1, His-tag , protein gel

Recombinant METTL11A / NTMT1, His-tag , protein gel
(Click to enlarge and view details)

MALDI-TOF Assay for METTL11A activity

MALDI-TOF Assay for METTL11A activity
(Click to enlarge and view details)