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Recombinant Proteins & Enzymes

Recombinant Mononucleosomes H4K20me3 (MLA)

Catalog No: 81080 Format: 20 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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Methylated Lysine Analog

Recombinant Mononucleosomes H4K20me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ϒ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

for Recombinant Mononucleosomes H4K20me3 (MLA)Contents

Recombinant Mononucleosomes H4K20me3 (MLA) (20 µg protein + 20 µg DNA) are supplied in 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 2 mM DTT and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant Mononucleosomes H4K20me3 (MLA)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Recombinant Histone H4K20me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine γ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112. 

for Recombinant Mononucleosomes H4K20me3 (MLA)Application Notes

Recombinant Mononucleosomes H4K20me3 (MLA) for analysis of histone modifications, or to generate chromatin in vitro. 

for Recombinant Mononucleosomes H4K20me3 (MLA)Protein Details

Recombinant Mononucleosomes H4K20me3 (MLA) consist of a 167 bp of 601 DNA with no tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3 that includes amino acids 1-136 (end) (accession number NP_003520.1), and H4 trimethyl Lys 20 (H4K20me3) (MLA) that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is 108 kDa. 

 
Recombinant Mononucleosomes H4K20me3 (MLA) SDS-PAGE

Recombinant Mononucleosomes H4K20me3 (MLA) SDS-PAGE
13% SDS-PAGE Coomassie staining
MW: 108 kDa
Purity: >95% 

Recombinant Mononucleosomes H4K20me3 (MLA) agarose gel

Recombinant Mononucleosomes H4K20me3 (MLA) agarose gel
Recombinant Mononucleosomes H4K20me3 (MLA) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H4K20me3. Intact mononucleosomes H4K20me3 migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrap histone octamers to form nucleosomes. 

Western Blot analysis for Recombinant Mono-nucleosomes H4K20me3 (MLA)

Western Blot analysis for Recombinant Mono-nucleosomes H4K20me3 (MLA)
Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H4K20me3 (MLA) (Lane 2) were detected with anti-H4K20me3 antibody (Cat. No. 39671) and anti-H3 antibody (Cat. No. 61475), respectively. H3 was detected as loading control. Only Recombinant Mononucleosomes H4K20me3 (MLA) can be detected by H4K20me3 antibody.

for Recombinant Mononucleosomes H4K20me3 (MLA)Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Mononucleosomes H4K20me3 (MLA)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant Mononucleosomes H4K20me3 (MLA)
 

Data Thumbnails

Recombinant Mononucleosomes H4K20me3 (MLA) SDS-PAGE

Recombinant Mononucleosomes H4K20me3 (MLA) SDS-PAGE
(Click to enlarge and view details).

Recombinant Mononucleosomes H4K20me3 (MLA) agarose gel

Recombinant Mononucleosomes H4K20me3 (MLA) agarose gel
(Click to enlarge and view details).

Western Blot analysis for Recombinant Mono-nucleosomes H4K20me3 (MLA)

Western Blot analysis for Recombinant Mono-nucleosomes H4K20me3 (MLA)
(Click to enlarge and view details).