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Recombinant Proteins & Enzymes

Recombinant RXR-α (RXRA) protein

Catalog No: 81082 Format: 20 µg $395 Buy Now
Catalog No: 81782 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant RXR-a (RXRA) proteinContents

Recombinant RXR-α (RXRA) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant RXR-a (RXRA) proteinBackground

RXR-α or so called RXRA (Retinoid X Receptor Alpha), also known as NR2B1, is a receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5-AGGTCA-3 sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. 

for Recombinant RXR-a (RXRA) proteinApplication Notes

This protein is suitable for use in protein-protein interaction, in vitro transcription assay, binding assay.

for Recombinant RXR-a (RXRA) proteinProtein Details

Recombinant human RXR-α (RXRA) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_002948.1) with an N-terminal FLAG tag. The molecular weight of the protein is 52.1 kDa. 

 
Recombinant RXR-α (RXRA) protein gel

Recombinant RXR-α (RXRA) protein gel
10% SDS-PAGE Coomassie staining
MW: 52.1 kDaPurity: >90% 

HTRF assay for RXR-α (RXRA) activity

HTRF assay for RXR-α (RXRA) activity
1 µM dsDNA oligos (DNA sequence: 5’-ATAGGTCACGGAGAGGTCAGC-3’) were incubated with different concentrations of protein in 10 µl binding system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hour, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

HTRF assay for RXR-α (RXRA) activity

HTRF assay for RXR-α (RXRA) activity
1 µM dsDNA oligos (DNA sequence: 5’-ATAGGTCACGGAGAGGTCAGC-3’) were incubated with different concentrations of protein in 10 µl binding system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hour, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

for Recombinant RXR-a (RXRA) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant RXR-a (RXRA) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant RXR-α (RXRA) protein
 

Data Thumbnails

Recombinant RXR-α (RXRA) protein gel

Recombinant RXR-α (RXRA) protein gel
(Click to enlarge and view details).

HTRF assay for RXR-α (RXRA) activity

HTRF assay for RXR-α (RXRA) activity
(Click to enlarge and view details).

HTRF assay for RXR-α (RXRA) activity

HTRF assay for RXR-α (RXRA) activity
(Click to enlarge and view details).