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Recombinant Proteins & Enzymes

Recombinant PDK1 protein

Catalog No: 81090 Format: 20 µg $395 Buy Now
Catalog No: 81790 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PDK1 proteinContents

Recombinant PDK1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.

for Recombinant PDK1 proteinBackground

PDK1 (Pyruvate Dehydrogenase Kinase 1), also known as PDHK1, is a serine/threonine kinase. It plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. PDK1 plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. Its activity can be enhanced by binding to the pyruvate dehydrogenase subunit DLAT, inhibited by AZD7545 (this compound interferes with DLAT binding and thereby inhibits kinase activity). Its activity can also be inhibited by dichloroacetate and radicicol.

for Recombinant PDK1 proteinApplication Notes

This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Kinase Activity Assay Conditions: 1 µM STK S3 substrate was incubated with different concentrations of PDK1 protein in a 10 µl reaction system containing 1X Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT, and 100 µM ATP for 1 hour. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.

for Recombinant PDK1 proteinProtein Details

Recombinant human PDK1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_002604.1) with an N-terminal FLAG tag. The molecular weight of the protein is 64.8 kDa.

 
Recombinant PDK1 protein gel

Recombinant PDK1 protein gel
10% SDS-PAGE Coomassie staining
MW: 64.8 kDa
Purity: >90%

HTRF assay for PDK1 activity

HTRF assay for PDK1 activity
STK S3 substrated was incubated with varying concentrations of PDK1 protein in a reaction system containing Enzymatic Buffer, MgCl2, DTT and ATP for 1 hr at RT. HTRF KinASE STK assay was used to detect the enzymatic activity.

for Recombinant PDK1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PDK1 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant PDK1 protein
 

Data Thumbnails

Recombinant PDK1 protein gel

Recombinant PDK1 protein gel
(Click to enlarge and view details)

HTRF assay for PDK1 activity

HTRF assay for PDK1 activity
(Click to enlarge and view details)