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Recombinant Proteins & Enzymes

Recombinant MLLT1 / ENL (1-148) protein

Catalog No: 81098 Format: 100 µg $395 Buy Now
Catalog No: 81898 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant MLLT1 / ENL (1-148) proteinContents

Recombinant MLLT1 / ENL (1-148) protein is supplied in 25 mM Tris- HCl pH 8.0, 300 mM NaCl, 10% glycerol and 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant MLLT1 / ENL (1-148) proteinBackground

MLLT1 / ENL (Myeloid/Lymphoid Or Mixed-Lineage Leukemia; Translocated To, 1), also known as YEATS1 or LTG19, is a component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.

for Recombinant MLLT1 / ENL (1-148) proteinApplication Notes

Recombinant MLLT1 / ENL (1-148) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Binding Assay Conditions: 3 µM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT1 / ENL (1-148) protein in 10 µl reaction system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hour, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. 

for Recombinant MLLT1 / ENL (1-148) proteinProtein Details

Recombinant human MLLT1 / ENL (1-148) that includes amino acids 1-148 (accession number NP_005925.2) was expressed in E. coli cells with an N-terminal 6×His tag and a C-terminal Flag tag. The molecular weight of the protein is 21.9 kDa.

 
Recombinant MLLT1 / ENL (1-148) protein gel

Recombinant MLLT1 / ENL (1-148) protein gel
12.5% SDS-PAGE Coomassie staining
MW: 21.9 kDa
Purity: >70%

Recombinant MLLT1 / ENL (1-148) activity assay

Recombinant MLLT1 / ENL (1-148) activity assay
H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT1 / ENL (1-148) protein in reaction buffer for 1 hour, then FLAG antibody and SA-XL665 mixture was added to each reaction system for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection. 

Recombinant MLLT1 / ENL (1-148) activity assay

Recombinant MLLT1 / ENL (1-148) activity assay
H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT1 / ENL (1-148) protein in reaction buffer for 1 hour, then FLAG antibody and SA-XL665 mixture was added to each reaction system for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection. 

for Recombinant MLLT1 / ENL (1-148) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant MLLT1 / ENL (1-148) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant MLLT1 / ENL (1-148) protein
 

Data Thumbnails

Recombinant MLLT1 / ENL (1-148) protein gel

Recombinant MLLT1 / ENL (1-148) protein gel
(Click to enlarge and view details)

Recombinant MLLT1 / ENL (1-148) activity assay


Recombinant MLLT1 / ENL (1-148) activity assay
(Click to enlarge and view details)

Recombinant MLLT1 / ENL (1-148) activity assay


Recombinant MLLT1 / ENL (1-148) activity assay
(Click to enlarge and view details)