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Recombinant Proteins & Enzymes

Recombinant YEATS2 (200-340) protein

Catalog No: 81099 Format: 100 µg $395 Buy Now
Catalog No: 81899 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant YEATS2 (200-340) proteinContents

Recombinant YEATS2 (200-340) protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol and 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant YEATS2 (200-340) proteinBackground

YEATS2 (YEATS Domain Containing 2), is a component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. YEATS2 specifically recognizes and binds histone H3 crotonylated at ‘Lys-27’ (H3K27cr). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors.

for Recombinant YEATS2 (200-340) proteinApplication Notes

Recombinant YEATS2 (200-340) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Binding Assay Conditions: 3 µM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in a 10 µl reaction system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hour, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. 

for Recombinant YEATS2 (200-340) proteinProtein Details

Recombinant human YEATS2 (200-340) that includes amino acids 200-340 (accession number NP_001338299.1) was expressed in E. coli cells with an N-terminal 6×His tag and a C-terminal Flag tag. The molecular weight of the protein is 21.5 kDa.

 
Recombinant YEATS2 (200-340) protein gel

Recombinant YEATS2 (200-340) protein gel
12.5% SDS-PAGE Coomassie staining
MW: 21.5 kDa
Purity: >80%

Recombinant YEATS2 (200-340) protein activity assay

Recombinant YEATS2 (200-340) protein activity assay
H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in reaction buffer for 1 hour, then FLAG antibody and SA-XL665 mixture was added to each reaction system for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. 

Recombinant YEATS2 (200-340) protein activity assay

Recombinant YEATS2 (200-340) protein activity assay
H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in reaction buffer for 1 hour, then FLAG antibody and SA-XL665 mixture was added to each reaction system for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. 

for Recombinant YEATS2 (200-340) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant YEATS2 (200-340) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant YEATS2 (200-340) protein
 

Data Thumbnails

Recombinant YEATS2 (200-340) protein gel

Recombinant YEATS2 (200-340) protein gel
(Click to enlarge and view details)

Recombinant YEATS2 (200-340) protein activity assay

Recombinant YEATS2 (200-340) protein activity assay
(Click to enlarge and view details)

Recombinant YEATS2 (200-340) protein activity assay

Recombinant YEATS2 (200-340) protein activity assay
(Click to enlarge and view details)