Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
    
Recombinant Proteins & Enzymes

Recombinant PRDM9 (191-415) protein

Catalog No: 81118 Format: 50 µg $395 Buy Now
Catalog No: 81818 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant PRDM9 (191-415) proteinContents

Recombinant PRDM9 protein is supplied at a concentration of 0.4 µg/µl in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol and 0.5 mM TCEP.

for Recombinant PRDM9 (191-415) proteinBackground

PRDM9 (PR Domain Containing 9) is a Histone methyltransferase that specifically trimethylates Lys-4 of histone H3 during meiotic prophase and is essential for proper meiotic progression. PRDM9 has the ability to mono- and dimethylate Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase (By similarity).

for Recombinant PRDM9 (191-415) proteinApplication Notes

This protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 1 µM histone peptide H3 (1-21aa) was incubated with different concentrations of PRDM9 protein in 10 µl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 3 hours, then 10 µl H3K4me3 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

for Recombinant PRDM9 (191-415) proteinProtein Details

Recombinant PRDM9 protein corresponding to amino acids 191-415 of human PRDM9 (accession number NP_064612.2) was expressed in E.coli cells with an N-terminal GST tag. The molecular weight of this protein is 52 kDa.

 
Recombinant PRDM9 protein

Recombinant PRDM9 protein
10% SDS-PAGE Coomassie staining
MW: 52 kDa
Purity: > 70%

HTRF assay for PRDM9 (191-415) activity

HTRF assay for PRDM9 (191-415) activity
1 µM histone peptide H3 (1-21) was incubated with PRDM9 (191-415) in reaction buffer for 3 hours at room temperature. HTRF assay was used for detection. H3K4me3 antibody was used to detect reaction product.

HTRF assay for PRDM9 (191-415) activity

HTRF assay for PRDM9 (191-415) activity
1 µM histone peptide H3 (1-21) was incubated with PRDM9 (191-415) in reaction buffer for 3 hours at room temperature. HTRF assay was used for detection. H3K4me3 antibody was used to detect reaction product.

for Recombinant PRDM9 (191-415) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PRDM9 (191-415) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant PRDM9 (191-415) protein
 

Data Thumbnails

Recombinant PRDM9 protein

Recombinant PRDM9 protein
(Click to enlarge and view details)

HTRF assay for PRDM9 (191-415) activity

HTRF assay for PRDM9 (191-415) activity
(Click to enlarge and view details)

HTRF assay for PRDM9 (191-415) activity

HTRF assay for PRDM9 (191-415) activity
(Click to enlarge and view details)