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Recombinant Proteins & Enzymes

Recombinant NgTet1 (1-321) protein

Catalog No: 81148 Format: 100 µg $395 Buy Now
Catalog No: 81848 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Naegleria

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for Recombinant NgTet1 (1-321) proteinContents

Recombinant NgTet1 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10% glycerol, and 0.5 mM TCEP.

for Recombinant NgTet1 (1-321) proteinBackground

Ten Eleven Translocation (TET) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine (5-hmC). 5-hmC can be further oxidized into 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for iinitiating the enzymatic deamination process leading to cytosine demethylation.

The Tet family of dioxygenases is widely distributed across the tree of life, including in the heterolobosean amoeboflagellate Naegleria gruberi . NgTet1 (Naegleria Tet-like protein) is a homologue of mammalian Tet proteins. Like mammalian Tet proteins, NgTet1 acts on 5-mC and generates 5-hmC, 5-fC and 5-caC.

for Recombinant NgTet1 (1-321) proteinApplication Notes

Recombinant NgTet1 protein is suitable for use in cytosine oxygenase assays, 5-mC sequencing, enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant NgTet1 (1-321) proteinProtein Details

Recombinant NgTet1 protein was expressed in E. coli cells as the full length protein (accession number XP_002667965.1) with an N-terminal 6xHis tag. The molecular weight of NgTet1 is 38.7 kDa.

 
Recombinant NgTet1 protein

Recombinant NgTet1 protein
10% SDS-PAGE Coomassie staining
MW: 38.7 kDa
Purity: >85%

Recombinant NgTet1 activity assay

Recombinant NgTet1 activity assay
1 µg of 30 nt ssDNA oligos with a 5mCpG site were incubated with different concentrations of NgTet1 in a reaction system at 34°C for 1 hour. Sample was concentrated to 10 µl, and 1 µl was spotted onto nylon membrane. 5hmC Ab (Cat. No. 39769) and 5-caC Ab (Cat. No. 61225) were used to detect products. NgTet1 activity assay was detected by Dot-Blot..

for Recombinant NgTet1 (1-321) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant NgTet1 (1-321) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant NgTet1 (1-321) protein
 

Data Thumbnails

Recombinant NgTet1 protein

Recombinant NgTet1 protein
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Recombinant NgTet1 activity assay

Recombinant NgTet1 activity assay
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