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Recombinant Proteins & Enzymes

Recombinant Mononucleosomes H3T3ph (EPL) - biotin

Catalog No: 81162 Format: 20 µg $445 Buy Now
Catalog No: 81862 Format: 1 mg $6,675 Buy Now
Expressed In: E. coli Protein Species: Human

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Expressed Protein Ligation

Recombinant Mononucleosomes H3T3ph (EPL) - biotin has been generated using the patented Expressed Protein Ligation (EPL) technology. EPL can be used to generate methylated, acetylated or phosphorylated histones. In EPL, the histone globular domain is ligated to a peptide that contains the N-terminal histone tail with the desired site-specific modification. The ligation reaction maintains the native histone bonds, and the resulting recombinant protein more closely mimics natural histones.

for Recombinant Mononucleosomes H3T3ph (EPL) - biotinContents

Recombinant Mononucleosomes H3T3ph (EPL) – biotin (20 µg protein + 20 µg DNA) are supplied at a protein concentration of 0.51 mg/ml in 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 2 mM DTT, and 20% glycerol.

for Recombinant Mononucleosomes H3T3ph (EPL) - biotinBackground

Western Blot analysis for Recombinant Mononucleosomes H3T3ph (EPL) – biotin
Recombinant Mononucleosomes H3T3ph (EPL) - biotin (Lane 1) and unmodified nucleosomes (Lane 2) were detected with H3T3ph antibody and H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3T3ph (EPL) - biotin can be detected by H3T3ph antibody.

for Recombinant Mononucleosomes H3T3ph (EPL) - biotinApplication Notes

Recombinant Mononucleosomes H3T3ph (EPL) - biotin are suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant Mononucleosomes H3T3ph (EPL) - biotinProtein Details

Recombinant Mononucleosomes H3T3ph (EPL) - biotin consist of a 167 bp of 601 DNA with 5’ biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NP_003503.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end) (accession number NP_066403.2) with phosphorylation at threonine 3, and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is ~108 kDa.

 
Recombinant Mononucleosomes H3T3ph (EPL) - biotin SDS PAGE gel

Recombinant Mononucleosomes H3T3ph (EPL) - biotin SDS PAGE gel
13% SDS-PAGE, stained with Coomassie blue
Purity: ≥ 92%

Recombinant Mononucleosomes H3T3ph (EPL) – biotin, DNA gel

Recombinant Mononucleosomes H3T3ph (EPL) – biotin, DNA gel
Mononucleosomes H3T3ph (EPL) - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact mononucleosome H3T3ph - biotin. Lane 3: 601 DNA. Intact mononucleosome H3T3ph - biotin migrated higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.

Western Blot analysis for Recombinant Mononucleosomes H3T3ph (EPL) – biotin

Western Blot analysis for Recombinant Mononucleosomes H3T3ph (EPL) – biotin
Recombinant Mononucleosomes H3T3ph (EPL) - biotin (Lane 1) and unmodified nucleosomes (Lane 2) were detected with H3T3ph antibody and H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3T3ph (EPL) - biotin can be detected by H3T3ph antibody.

for Recombinant Mononucleosomes H3T3ph (EPL) - biotinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Mononucleosomes H3T3ph (EPL) - biotinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant Mononucleosomes H3T3ph (EPL) - biotin
 

Data Thumbnails

Recombinant Mononucleosomes H3T3ph (EPL) - biotin SDS PAGE gel

Recombinant Mononucleosomes H3T3ph (EPL) - biotin SDS PAGE gel
(Click to enlarge and view details)

Recombinant Mononucleosomes H3T3ph (EPL) – biotin, DNA gel

Recombinant Mononucleosomes H3T3ph (EPL) – biotin, DNA gel
(Click to enlarge and view details)

Western Blot analysis for Recombinant Mononucleosomes H3T3ph (EPL) – biotin

Western Blot analysis for Recombinant Mononucleosomes H3T3ph (EPL) – biotin
(Click to enlarge and view details)