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Recombinant Proteins & Enzymes

Recombinant YTHDC1 protein

Catalog No: 81176 Format: 20 µg $405 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant YTHDC1 proteinContents

Recombinant YTHDC1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.

for Recombinant YTHDC1 proteinBackground

YTHDC1 (YTH Domain Containing 1), is a regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDC1 acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. It specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. YTHDC1 may also regulate alternative splice site selection.

for Recombinant YTHDC1 proteinApplication Notes

Recombinant YTHDC1 protein is suitable for use in the study of binding assay, inhibitor screening, and selectivity profiling.

for Recombinant YTHDC1 proteinProtein Details

Recombinant YTHDC1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001026902.1) with an N-terminal Flag tag. The molecular weight of the protein is 85.6 kDa

 
Recombinant YTHDC1 protein gel

Recombinant YTHDC1 protein gel
7.5% SDS-PAGE Coomassie staining
MW: 85.6 kDa
Purity: >85%

for Recombinant YTHDC1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant YTHDC1 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Technical Data Sheet

Recombinant YTHDC1 protein
 

Data Thumbnails

Recombinant YTHDC1 protein gel

Recombinant YTHDC1 protein gel
(Click to enlarge and view details)