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Antibodies

AbFlex® Histone H3S10ph antibody (rAb)

Catalog No: 91131 Format: 100 µg $375 Buy Now
Catalog No: 91132 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Human

Applications

Western Blot Validated Dot Blot Validated

for AbFlex Histone H3S10ph antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 – 2 µg/ml
DB: 2 µg/ml

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61623.

for AbFlex Histone H3S10ph antibody (rAb)Immunogen

This antibody was raised against a peptide containing phospho-serine 10 of Histone H3.

for AbFlex Histone H3S10ph antibody (rAb)Buffer

Purified IgG in 140mM Hepes, pH 7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol.

 
AbFlex<sup>®</sup> Histone H3S10ph antibody (rAb) tested by Western blot.

AbFlex® Histone H3S10ph antibody tested by Western blot.
20 µg of HeLa cell extracts (Lane 1 – untreated, Lane 2 – Colcemid treated) were run on SDS-PAGE and probed with AbFlex Histone H3S10ph antibody at 0.5 µg/ml.

AbFlex<sup>®</sup> Histone H3S10ph antibody (rAb) tested by dot blot analysis.

AbFlex® Histone H3S10ph antibody specificity is shown by Dot Blot.
Recombinant peptides were spotted onto PVDF as follows: Column 1 - Histone H3S10ph; Column 2 - Histone H3S10 (non-phosphorylated); Column 3 - Histone H3.1S28ph; Column 4 - Histone H3.1S28 (non-phosphorylated); Column 5 - Histone H3.3S31ph; Column 6 - Histone H3.3S31ph (non-phosphorylated); Column 7- Histone H3.3S28ph. The membrane was probed with AbFlex® Histone H3S10ph antibody at 2 µg/ml.

for AbFlex Histone H3S10ph antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, AbFlex® Histone H3S10ph antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO (Chinese Hamster Ovary) cells.

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is strongly phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.

for AbFlex Histone H3S10ph antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Histone H3S10ph antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

AbFlex® Histone H3S10ph antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Histone H3S10ph antibody (rAb) tested by Western blot.

Western blot of AbFlex® Histone H3S10ph antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> Histone H3S10ph antibody (rAb) tested by dot blot analysis.

Dot blot of AbFlex® Histone H3S10ph antibody (rAb).
(Click image to enlarge and see details.)