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Antibodies

AbFlex® JMJD2A antibody (rAb)

Aliases: KDM4A, JMJD2, JHDM3A, TDRD14A
Catalog No: 91143 Format: 100 µg $375 Buy Now
Catalog No: 91144 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:130 kDa
Reactivity:Human

Applications

Western Blot Validated Enzyme-linked Immunosorbent Assay Validated

for AbFlex JMJD2A antibody (rAb)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution
Bead-based ELISA: 40 - 600 ng/ml

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for AbFlex JMJD2A antibody (rAb)Immunogen

This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.

for AbFlex JMJD2A antibody (rAb)Buffer

Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide.

AbFlex™ recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 39815.

 
AbFlex<sup>®</sup> JMJD2A antibody (rAb) tested by Western blot.

AbFlex® JMJD2A antibody (rAb) tested by Western blot.
HeLa cell nuclear extract (20 µg) probed with AbFlex® JMJD2A antibody (rAb) at a dilution of 2 µg/ml.

AbFlex<sup>®</sup> JMJD2A antibody (rAb) tested by Western blot.

AbFlex® JMJD2A antibody (rAb) tested by Luminex bead-based specificity analysis.
Luminex bead-based specificity analysis was used to confirm the specificity of AbFlexTM JMJD2A antibody (rAb) antibody for JMJD2A. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexTM JMJD2A antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument.

Luminex® is a registered trademark of Luminex Corporation.

for AbFlex JMJD2A antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

AbFlex® JMJD2A antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

for AbFlex JMJD2A antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex JMJD2A antibody (rAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® JMJD2A antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> JMJD2A antibody (rAb) tested by Western blot.

Western blot of AbFlex® JMJD2A antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> JMJD2A antibody (rAb) tested by Western blot.

Western blot of AbFlex® JMJD2A antibody (rAb).
(Click image to enlarge and see details.)