Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
    
Antibodies

AbFlex® Suz12 antibody (rAb)

Catalog No: 91163 Format: 100 µg $375 Buy Now
Catalog No: 91164 Format: 10 µg $75 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:95 kDa
Reactivity:Human

Applications

Immunoprecipitation Validated

for AbFlex Suz12 antibody (rAb)Application Notes


Validated Applications:
Immunoprecipitation (IP): 2 µg per IP

Note: For optimal results, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for detection of Suz12.

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39877.

for AbFlex Suz12 antibody (rAb)Immunogen

This antibody was raised against full length human SUZ12 (suppressor of zeste 12 homolog (Drosophila))

for AbFlex Suz12 antibody (rAb)Buffer

140mM Hepes, pH 7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol.

 
AbFlex<sup>®</sup> Suz12 antibody (rAb) tested by immunoprecipitation.

AbFlex® Suz12 antibody (rAb) tested by Immunoprecipitation
300 µg of HeLa cell nuclear extract was immunoprecipitated with 5 µg of either AbFlex Suz12 antibody (Lane 1) or Mouse IgG (Lane 2). Immunoprecipitated proteins were run on SDS-PAGE and probed using a polyclonal antibody specific for Suz12 (Cat. No. 39357) at 2 µg/ml. On the gel, antibody heavy chains (50 kDa) and light chains (25 kDa) are visible.

for AbFlex Suz12 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, AbFlex® Suz12 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3 dimethyl Lys27 and Histone H3 trimethyl Lys27), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use AbFlex® Suz12 antibody (rAb).

for AbFlex Suz12 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Suz12 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Suz12 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Suz12 antibody (rAb) tested by immunoprecipitation.

Immunoprecipitation of AbFlex® Suz12 antibody (rAb).
(Click image to enlarge and see details.)